Дерамиоцел

Prop. INN (наименование, предложенное ВОЗ)

allogeneic cardiosphere-derived cells obtained from cultured human donor myocardial tissue. The tissue is grown in culture until explant derived cells emerge. These cells are then frozen into a Master cell bank until seeded in ultra-low attachment flasks in the presence of bovine serum where they spontaneously form spherical three-dimensional cell clusters termed cardiospheres., The cardiospheres are then disrupted to form adherent single cells, which are further expanded onto fibronectin-coated flasks in the presence of bovine serum with the cells undergoing a range of 5-10 population doubling (PDLs) to be cryopreserved until use.
The cardiosphere-derived cells (CDC) are CD105 high (>90%) and CD45 low (<10%). They retain antifibrotic activity as measured by assessing collagen 1A and 3A messenger RNA (mRNA) levels in fibroblasts cultured with conditioned media collected from CDCs and are evaluated by bioinformatics analyses where the desired mRNA expression profile is correlated with known clinically potent samples lots

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