Марнетеграген аутотемцел

Rec. INN (наименование, зарегистрированное ВОЗ)

autologous CD34+ hematopoietic cells obtained from mobilised peripheral blood, transduced with a selfinactivating, non-replicating lentiviral vector expressing human integrin β2 (ITGB2, CD18) under the control of a cathepsin G (CatG)/c-Fes chimeric promoter; the construct is flanked by 5' and 3' long terminal repeats (LTRs) and also contains a ψ packaging signal, a Rev response element (RRE), a central polypurine tract (cPPT) sequence and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). The vector is pseudotyped with the envelope glycoprotein (G) of vesicular stomatitis virus (VSV). The CD34+ cell population was enriched using immunomagnetic microbeads, and the cells cultured in bags coated with a fragment of recombinant human fibronectin with medium containing stem cell factor (SCF), FMS-related tyrosine kinase 3 ligand (Flt-3L), thrombopoietin (TPO), and interleukin 3 (IL-3 ). Myeloid differentiated cells generated from the transduced CD34+ cells bind soluble intercellular adhesion molecule-1 (sICAM-1) upon activation.

Поделиться этой страницей