Рилдинадстроцел

Rec. INN (наименование, зарегистрированное ВОЗ)

allogeneic mesenchymal stromal cells (MSC) prepared from subcutaneous adipose tissue of healthy donors, collected by liposuction.
The adipose tissue is digested and the released cells placed in culture media containing bovine serum albumin (BSA), fetal bovine serum (FBS), hydrocortisone, recombinant human epidermal growth factor (rh- EGF), recombinant human fibroblast growth factor (rh-FGF), recombinant human transferrin and insulin, heparin and L-alanyl-L-glutamine. The cells are frozen to generate a master cell bank (MCB) at an early passage, and then resuscitated and culture-expanded. The cells express mesenchymal stromal cell marker cluster of differentiation 73 (CD73), CD90, and CD105 (>80%) and do not express human leukocyte antigen-DR isotype (HLA DR), CD34, CD11b, CD19, and CD45 (<5%). Resting cells are expressing CD274 (PDL1, >2%). The activated cells secrete vascular endothelial growth factor (VEGF) and upregulate expression of indoleamine 2,3-dioxygenase 1 (IDO1) mRNA when activated in vitro.

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