Трокулеуцел

Rec. INN (наименование, зарегистрированное ВОЗ)

autologous natural killer (NK) cells isolated from peripheral blood leukapheresis of individual healthy donors. The leukapheresis material is enriched for CD56+ cells by positive immunoselection and re-suspended in media containing fetal bovine serum (FBS), interleukin-2 (IL-2), and interleukin-21 (IL-21). The cells are initially cultured in media containing IL-2 and two different irradiated feeder cell lines and then cultured with IL-2 alone to induce cell expansion.
The final cell population consists of CD3-CD56+ NK cells (≥80%), with highly expressed activating NK cell receptors [NKp30, NKp44 and NKp46, C-type lectin-like receptor (NKG2 family of receptors including NKG2C and NKG2D)], and other activating receptors such as CD16 and CD244. The cells also upregulate expression of perforin-1, granzyme, tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ) and CD107a, and demonstrate cytotoxicity against K562 myeloid leukemia cells in vitro (≥50%)

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