Видиденцел

Rec. INN (наименование, зарегистрированное ВОЗ)

cell-cycle arrested dendritic cells (DCs) derived from the cell line DCOne, which originated from MUTZ-3 cells that arose from mononuclear blasts isolated from a patient with acute myelomonocytic leukaemia. The cells are initially grown in media containing granulocyte-macrophage colony-stimulating factor (GM- CSF), followed by differentiation in the presence of granulocyte- macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor alpha (TNF-α), interleukin 4 (IL-4) and mitoxantrone. Finally, the cells are matured in the presence of media containing prostaglandin-E2 (PGE2), TNF-α, and IL-1β. After cryopreservation the cells are irradiated to induce cell-cycle arrest. The final cell suspension contains >70% viable cells, of which >70% HLA-DR/DP/DQ-positive, >70% HLA-ABC- positive, >30% CD1a (DC differentiation marker)-positive, >30% CD83 (DC maturation marker)-positive, and which express co-stimulatory molecules highly expressed by mature DCs, i.e., CD40 (>60%), CD80 (>50%) and CD86 (>70%). The preparation also stimulates T lymphocytes in a mixed lymphocyte reaction (MLR).

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